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DNA/RNA Dilution Calculator

Dilute a DNA or RNA stock to exactly the concentration and volume you need. Use it when preparing template for PCR, samples for sequencing or ligation, or working stocks from a concentrated prep.

Enter your stock concentration, the concentration you need and the final volume. We'll tell you exactly how much stock and diluent to mix.

The concentration of the tube you are diluting from.

The concentration you want to end up with.

How much diluted solution you need in total.

Dilute with
Enter your values and press Calculate to see how much stock and water to mix.

How it's calculated

C1 × V1 = C2 × V2

Stock volume (V1) = C2 × V2 ÷ C1

Diluent = V2 − V1

C1 is the stock concentration, C2 the concentration you want and V2 the final volume. Stock and target must be in the same kind of unit (mass or molar); 1 µg/mL equals 1 ng/µL.